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I am a recent MSc Biotechnology graduate from Amity University, with expertise in toxicity assessments and comprehensive analytical techniques. I am highly motivated to pursue a PhD focused on studying environmental pollutants.

Basics

Name Bharathkumar Rajagopal
Diploma Msc Biotechnology
Email i.bharath@outlook.com
Phone (+91) 8072253737
Url https://bharathkumar-rajagopal.github.io/
Summary Prospective PhD candidate

Work

  • 2024.07 - present
    Independent Researcher (Under Scholarly Mentorship)
    Engaged in independent research in environmental toxicology under the scholarly mentorship of Dr. Boobal Rangaswamy, Department of Biotechnology, PSG College of Arts & Science, contributing substantively to the development and authorship of scientific manuscripts intended for peer-reviewed publication.
    • Ecotoxicology, Independent Research, Scientific Writing, Manuscript Preparation
  • 2024.01 - 2024.05
    Dissertation Trainee
    CSIR - Indian Institute of Toxicology Research, Lucknow
    Thesis Title: In Vitro Toxicity Assessment of Polystyrene Nanoplastics in Chinese Hamster Lung fibroblast cells (V-79)
    A concentration-dependent increase in irregular cell morphology was observed, with decreased cell viability in the Trypan Blue and Comet assays. ROS assay indicated varied response, suggesting alternative toxicity pathways at higher PS NPs exposure.
    • Toxicology, Risk assessments, Mammalian cell culture establishment and maintainance, Cytotoxicity assays, Genotoxicity assays
  • 2023.07 - 2023.12
    Postgraduate Research Assistant
    Centre for Biotechnology and Biochemical Engineering, Amity Institute of Biotechnology, Amity University Uttar Pradesh.
    Project: Microbeads preparation with Bacterial Consortia
    The microbeads, synthesised using bacterial consortia that degrade pesticides, facilitate the storage and transport of these consortia for extended periods.
    • Microbial Culture Establishment and Maintainance, Microbead preparation and optimisation
  • 2023.06 - 2023.07
    Summer Research Intern
    Centre for Biotechnology and Biochemical Engineering, Amity Institute of Biotechnology, Amity University Uttar Pradesh.
    Project: Biogenic Synthesis of Silver Nanoparticles using Endophytic Bacteria Isolated from Andrographis paniculata and its Potential Applications as Anticancer and Antibacterial Agents
    Endophytic bacteria identified as Priestia megaterium were used to synthesize silver nanoparticles, which were characterized by UV-Visible Spectrophotometry, FTIR, and SEM. The nanoparticles showed significant antibacterial activity and reduction in cell viability in the Hep G2 cell line.
    • Biogenic synthesis of nanoparticle, Green synthesis, Microbial Culture Establishment and Maintainance, Time kill assay, SEM, FTIR

Volunteer

  • 2023.11 - present

    Noida, India

    Volunteer
    Liquid Trees
    Currently supporting ``Diatoms for a Cooler Tomorrow`` at Liquid Trees, a bioremediation startup. Join the movement to neutralize pollution and combat climate challenges. Let's make a real difference together!
    • Diatoms, CO2 reduction
  • 2023.09 - 2023.09

    Manesar, India

    Military Training Camp Ser – 262
    Amity Indian Military College (AIMC Manesar)
    Gained invaluable experience and skills through participation in a Military Training Camp. Developed discipline, leadership abilities, teamwork, and resilience under challenging conditions. This training enhanced my physical and mental stamina and instilled a strong sense of duty and commitment.
    • Discipline, Patriotism, Physical Training
  • 2023.08 - 2024.06

    Noida, India

    Student Coordinator
    Corporate Resource Center (CRC), Amity Institute of Biotechnology, Amity University Uttar Pradesh
    As a Corporate Resource Center (CRC) Student Coordinator at Amity Institute of Biotechnology (AIB), I focused on coordinating and organizing various programs. My role involved guiding students toward higher education opportunities in India and abroad through well-planned webinars and comprehensive guidance sessions.
    • Leadership, Team management, Event organisation
  • 2023.02 - 2023.05

    Noida, India

    Social Work Intern - Tutor
    Manjil Non-Governmental Organization
    Taught underprivileged children, focusing on their sustenance and growth within challenging geopolitical conditions.
    • Social Work, Tutoring
  • 2022.08 - 2024.06

    Noida, India

    Member
    Youth Forum Associated with Amity University Uttar Pradesh
    • Community Servise, Event organisation, Team work
  • 2019.08 - 2022.06

    Coimbatore, India

    Member
    Bio Beacons (Department Association) Associated with PSG College of Arts & Science
    • Sience, Group Discussion

Education

  • 2022.09 - 2024.07

    Noida, India

    Master of Science (MSc.)
    Amity Institute of Biotechnology, Amity University Uttar Pradesh
    Major: Biotechnology
    • Advanced Molecular Biology, Advanced Animal Biotechnology, Advance Applied Biostatistics for Biotechnologists, Molecular Cell Biology, Advanced Biochemistry, Advanced Genetics, Advanced Immunology, Enzymes and Enzyme Technology, Python, Applied Genomics and Proteomics, Advanced Microbiology
  • 2019.09 - 2022.07

    Coimbatore, India

    Bachelor of Science (BSc.)
    PSG College of Arts & Science, Bharathiar University
    Major: Biotechnology
    • Environmental Biotechnology, Molecular Biology, Recombinant DNA Technology, Chemistry of Biomolecules, Microbiology, Computer Applications in Biotechnology, Analytical Techniques, Metabolism, Biopharmaceuticals, Animal Biotechnology, Immunology

Awards

Certificates

Introduction to Professional Scientific Communication
National Programme on Technology Enhanced Learning (NPTEL), MoE, Government of India 2024-02-31
Certificate course on Bionanotechnology (CBNT)
Association of Indian Biologists (AIB) 2020-05-31
Certificate in Bioinformatics
PSG College of Arts & Science, Bharathiar University 2020-05-31
Human Molecular Genetics
National Programme on Technology Enhanced Learning (NPTEL), MoE, Government of India 2020-02-31
Yoga for Youth Empowerment
SKY Yoga 2018-06-01
Hands-on training on “HPLC and GC-MS/MS: Instrumentation, Operation and Application”
ICFRE - Institute of Forest Genetics and Tree Breeding 2018-02-28
Global Digital Corps (GDC) Field Ops Cadet
Pupilfirst and recognized by the United Nations 2018-01-01

Skills

Applied computational techniques
BLAST
PSIPRED
Biomolecular visualisation
SWISS-MODEL
Rasmol
Pymol
Molecular Biology
DNA isolation and purification
PCR
Agarose gel electrophoresis
SDS-PAGE
Western Blotting
Microbiology
Isolating pure culture
Culturing and plating
Staining and visualisation
sterilisation and fumigation
Toxicology
MTT
Neutral red dye uptake assay
LDH
Trypan blue dye exclusion assay
Genotoxicity Assay - Alkaline single-cell electrophoresis (comet assay)
Analytical techniques
Column chromatography
Affinity chromatography
GC-MS/MS
HPLC
Spectrophotometry
Dynamic Light Scattering (DLS)
Nanoparticle Tracking Analysis (NTA)
Language
Python (Foundation)
LaTeX

Languages

English
CEFR - C1 (Advanced)
Tamil
Native Speaker

References

Dr. Alok Kumar Pandey
Senior Principal Scientist - CSIR - Indian Institute of Toxicology Research, Lucknow
Dr. Ravi Kant Singh
Professor - Amity Institute of Biotechnology, Amity University Uttar Pradesh
Dr. Boobal Rangaswamy
Assistant Professor – Department of Biotechnology, PSG College of Arts & Science, Tamil Nadu